|
Designations:
|
DH82
|
|
Depositors:
|
The United States of America
|
|
Biosafety Level:
|
1
|
|
Shipped:
|
frozen
|
|
Medium & Serum:
|
See Propagation
|
|
Growth Properties:
|
adherent
|
|
Organism:
|
Canis familiaris
|
|
Morphology:
|
macrophage
|
|
Source:
|
Disease: malignant histiocytosis
|
|
Permits/Forms:
|
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
|
|
Age:
|
10 years
|
|
Gender:
|
male
|
|
Propagation:
|
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.
Temperature: 37.0°C
|
|
Subculturing:
|
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
|
|
Preservation:
|
culture medium 95%; DMSO, 5%
|
|
Related Products:
|
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
|
|
References:
|
22019: Dawson JE, Rikihisa Y. Growing Ehrlichia species in a continuous cell line. US Patent 5,192,679 dated Mar 9 1993
22675: Wellman ML, et al. A macrophage-monocyte cell line from a dog with malignant histiocytosis. In Vitro Cell. Dev. Biol. 24: 223-229, 1988. PubMed: 3350786
|
