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Sf9
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G Smith, C Cherry, MD Summers
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Biosafety Level:
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1
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frozen
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See Propagation
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mixed: adherent/suspension
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Spodoptera frugiperda (fall armyworm)
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epithelial
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Organ: ovary
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Isolation date: 1983
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transfection host (Roche FuGENE® Transfection Reagents
technology from amaxa)
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Nuclear polyhedrosis virus, Autographa californica
St. Louis encephalitis virus
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pupa
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female
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ATCC complete growth medium: Grace's Insect Medium with L-glutamine and supplemented with:
500 mg/L calcium chloride
2800 mg/L potassium chloride
3330 mg/L lactalbumin hydrolysate
3330 mg/L yeastolate
10% Heat-inactivated fetal bovine serum (previously tested for insect cell culture)
Atmosphere: air,
Temperature: 28.0°C
Growth Conditions: The recommended media are formulated for use without CO2. Omission of the yeastolate or lactalbumin hydrolysate will lead to poor performance by this line.
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Protocol: Gently resuspend cells in the spent culture medium by pipetting across the monolayer or by hitting the flask against the palm of your hand (the latter is only preferable when working with larger flasks).
Subcultivation Ratio: A subcultivation ratio of 1:5 or greater is recommended
Medium Renewal: Every 2 to 3 days
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Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
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22650: Vaughn JL, et al. The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro 13: 213-217, 1977. PubMed: 68913
23136: Zhang PF, et al. Susceptibility of the Sf9 insect cell line to infection with adventitious viruses. Biologicals 22: 205-213, 1994. PubMed: 7811453
23294: Smith GE, et al. Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector. Proc. Natl. Acad. Sci. USA 82: 8404-8408, 1985. PubMed: 3878519
32359: Hall MR, Gibson W. Cytomegalovirus assemblin: the amino and carboxyl domains of the proteinase form active enzym e when separately cloned and coexpressed in eukaryotic cells. J. Virol. 70: 5395-5404, 1996. PubMed: 8764050
32361: Bakker A, et al. Human T-cell leukemia virus type 2 Rex inhibits pre-mRNA splicing in vitro at an early stage of spliceosome formation. J. Virol. 70: 5511-5518, 1996. PubMed: 8764063
32693: Gibson W, et al. Cytomegalovirus "missing" capsid protein identified as heat-aggregable product of human cytomegalovirus UL46. J. Virol. 70: 7454-7461, 1996. PubMed: 8892863
32860: Filtz TM, et al. Purification and G protein subunit regulation of a phospholipase C-beta from Xenopus laevis oocytes. J. Biol. Chem. 49: 31121-31126, 1996. PubMed: 8940109
32882: Lindorfer MA, et al. G protein gamma subunits with altered prenylation sequences are properly modified when expressed in Sf9 cells. J. Biol. Chem. 271: 18582-18587, 1996. PubMed: 8702508
33023: Bruant SS, et al. N-terminal sequences contained in the Src homology 2 qand 3 domains of p120 GTPase-activating protein are required for full catalytic activity toward ras. J. Biol. Chem. 271: 5195-5199, 1996. PubMed: 8617802
33034: Stewart L, et al. Biochemical and biophysical analyses of recombinant forms of human topoisomerase I. J. Biol. Chem. 271: 7593-7601, 1996. PubMed: 8631793
33143: Kawabe J, et al. Soluble adenylyl cyclase from Spodoptera frugiperda (Sf9) cells. J. Biol. Chem. 271: 20132-20137, 1996. PubMed: 8702736
33160: Yasuda H, et al. Role of the prenyl group on the G protein gamma subunit in coupling trimeric G proteins to A1 adenosine receptors. J. Biol. Chem. 271: 18588-18595, 1996. PubMed: 8702509
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