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Sp2/mIL-6
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JF Harris
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Biosafety Level:
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1
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frozen
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Medium & Serum:
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See Propagation
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suspension
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Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma))
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lymphoblast
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Cell Type: hybridoma: B lymphocyte;
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mouse interleukin 6 (interleukin-6, IL-6)
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Note: These cells are distributed for research purposes only. Dr. Harris releases the line subject to the following: 1.) The Sp2/mIl-6 cells or any cells derived from SP2/mIL-6 or any antibody derived from it must not be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. 2.) Any proposed commercial use of these cells should first be negotiated with the London Regional Cancer Centre, 790 Commissioners Road, East, London, Ontario. N6A 4L6 Canada. 3.) In all papers reporting any use of these or derived clones a direct reference will be made to the original publication (J. Immunol. Methods 148: 199-207, 1992).
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Toronto Ontario, Canada
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H-2, d haplotype; Mus musculus, expressed
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ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
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fetal bovine serum to a final concentration of 10%
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1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
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0.05 mM 2-Mercaptoethanol
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Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 2 X 10(5) cells/ml and subculture before the cell density reaches 2 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 4 days (depending on cell density)
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
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23169: Harris JF, et al. Increased frequency of both total and specific monoclonal antibody producing hybridomas using a fusion partner that constitutively expresses recombinant IL-6. J. Immunol. Methods 148: 199-207, 1992. PubMed: 1373425
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